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Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
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JoVE Revista Biología del desarrollo
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

DOI:
10.3791/55904-v

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10:07 min

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May 26, 2017

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, ,
1College of Pharmacy,University of Minnesota

Capítulos

  • 00:05Título
  • 00:47Muscle Dissection and Single-cell Isolation
  • 03:02Cell Sorting
  • 07:03Post-sorting Analyses
  • 08:22Results: Distinct Characteristics of Type I and Type II Pericytes
  • 09:35Conclusion

Summary

Traducción Automática

Traducción Automática

This work describes a FACS-based protocol that allows for easy and simultaneous isolation of type I and type II pericytes from skeletal muscles.

Tags

Pericyte IsolationType I PericyteType II PericyteSkeletal MuscleFACSNestin-GFPCollagenaseTrypsin-EDTARed Blood Cell LysisCell SortingCell Suspension

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