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应用酵母雌激素筛检优选本科教学实验室的个人护理产品中的雌激素配体
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
DOI:

14:13 min

January 01, 2018

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Capítulos

  • 00:05Título
  • 01:02Preparing Reagents, Samples, and Extraction Controls
  • 03:23Subculturing Yeast and Preparing YES Plates
  • 07:18Processing YES Plates (Day 2 of the Assay)
  • 09:26Calculating LacZ Values
  • 10:28Interpolating Sample Estradiol Equivalents (EEQs) using 4-parameter Logistic Regression
  • 11:30Results: Yeast Estrogen Screen Quantifies Ligands in Personal Care Products (PCPs)
  • 12:52Conclusion

Summary

Traducción Automática

本文介绍了一个优化酵母雌激素屏幕的量化配体的个人护理产品 (杆), 结合雌激素受体α (ERα) 和/或β (er)。该方法采用了两种色度基质选择, 一个六天的冷冻孵化, 用于本科课程, 以及数据分析的统计工具。

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