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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Capítulos
- 00:05Título
- 00:55Recombinant Protein Production and Purification of rBC1531
- 04:47Removal of Affinity Tags by Thrombin Proteolysis
- 06:32Crystallization Screening and Optimization of rBC1531
- 07:30Colorimetric NTPase Assay
- 08:33Results: Purification, Characterization, and Crystallization of rBC1531
- 10:31Conclusion
Bacillus anthracis is the obligate pathogen of fatal inhalational anthrax, so studies of its genes and proteins are strictly regulated. An alternative approach is to study orthologous genes. We describe biophysicochemical studies of a B. anthracis ortholog in B. cereus, bc1531, requiring minimal experimental equipment and lacking serious safety concerns.
