/
/
A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Capítulos
- 00:05Título
- 00:46Infection Protocol to Generate Mtb/Macrophage Aggregate Structures
- 04:04Growth Inhibition Assay to Assess Drug Efficacy against Mtb Derived from Mtb/Macrophage Aggregates
- 06:31Quantification of Drug Efficacy Using the Resazurin Microtiter Assay
- 07:54Using the Resazurin Microtiter Assay to Measure Mtb Viability
- 09:52Conclusion
New models and assays that would improve the early drug development process for next-generation anti-tuberculosis drugs are highly desirable. Here, we describe a quick, inexpensive, and BSL-2 compatible assay to evaluate drug efficacy against Mycobacterium tuberculosis that can be easily adapted for high-throughput screening.
