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Immunology and Infection

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JoVE Journal Immunology and Infection
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
 

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

Article DOI: 10.3791/55390-v • 09:00 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 1, *1, *1
1Department of Neurology with Institute of Translational Neurology, University Hospital Münster
* These authors contributed equally

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Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.

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Lymphocyte Extravasation In Vitro Model Blood-brain Barrier Transmigration Assay Central Nervous System Inflammatory Diseases Therapeutic Approaches Rare Lymphocyte Populations Single Cell Level Blood-cerebrospinal Fluid Barrier Evans Blue Permeation Analysis Fibronectin Solution Human Brain Microvascular Endothelial Cells (HBMEC) Complete ECM-b Medium
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