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检测和罕见富集抗原特异性B细胞的表型和功能分析
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
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检测和罕见富集抗原特异性B细胞的表型和功能分析

11,184 Views

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09:25 min

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February 16, 2017

DOI:

10.3791/55382-v

DOI:
10.3791/55382-v

•

09:25 min
February 16, 2017

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, , , , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology,Colorado State University, 3Barbara Davis Center for Childhood Diabetes,University of Colorado School of Medicine, 4Department of Biomedical Research,National Jewish Health

Tags

Rare Antigen-specific B CellsMagnetic NanoparticlesPhenotypic AnalysisFunctional AnalysisImmunologyFlow CytometryDensity Gradient SeparationMononuclear CellsFc Gamma Receptor BlockingFluorescence-conjugated AntibodiesBiotinylated Antigen

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Smith, M. J., Packard, T. A., O'Neill, S. K., Hinman, R. M., Rihanek, M., Gottlieb, P. A., Cambier, J. C. Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function. J. Vis. Exp. (120), e55382, doi:10.3791/55382 (2017).

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