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JoVE Journal Developmental Biology
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
 

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Article DOI: 10.3791/55268-v • 08:21 min • March 16th, 2017
March 16th, 2017 •
1, 1
1Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania

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Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.

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Keywords: Human Pluripotent Stem Cells Trophoblastic Cells Bone Morphogenic 4 Nodal Signaling Pathways Placental Formation Extracellular Matrix Mouse Embryonic Fibroblast-conditioned Medium BFGF Dispase Differentiation Medium
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