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Intracellulær Farvning og flowcytometri at identificere lymfocytundergrupper inden Murine Aorta, Nyre og lymfeknuder i en model af hypertension
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JoVE Revista Inmunología y contagio
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Intracellulær Farvning og flowcytometri at identificere lymfocytundergrupper inden Murine Aorta, Nyre og lymfeknuder i en model af hypertension

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09:20 min

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January 28, 2017

DOI:

10.3791/55266-v

DOI:
10.3791/55266-v

•

09:20 min
January 28, 2017

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1Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics,Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy,Mansoura University

Tags

Intracellular StainingFlow CytometryLymphocyte SubsetsMurine AortaKidneyLymph NodesHypertensionAngiotensin IISingle-cell SuspensionImmune CellsCardiovascular DiseasePerfusionDissectionDensity Gradient Centrifugation

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Laroumanie, F., Dale, B. L., Saleh, M. A., Madhur, M. S. Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension. J. Vis. Exp. (119), e55266, doi:10.3791/55266 (2017).

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