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Um glóbulo vermelho simples método de lise para o estabelecimento de linhas celulares linfoblastóides B
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JoVE Revista Inmunología y contagio
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

Um glóbulo vermelho simples método de lise para o estabelecimento de linhas celulares linfoblastóides B

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09:49 min

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January 14, 2017

DOI:

10.3791/55191-v

DOI:
10.3791/55191-v

•

09:49 min
January 14, 2017

•

2 Views
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1Wuhan Institute of Virology,Chinese Academy of Sciences, 2Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences

Tags

B Lymphoblastoid Cell LinesB-LCLsImmortalization EfficiencyRed Blood Cell LysisEpstein-Barr VirusPBMCDensity Gradient CentrifugationEBV TransformationCell CultureCryopreservationVirus Particle ReleaseFiltrationWhole Blood

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Liu, X., Xu, C., Duan, Z. A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines. J. Vis. Exp. (119), e55191, doi:10.3791/55191 (2017).

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