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方法可视化和用透射电子显微镜分析膜蛋白的相互作用
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March 05, 2017
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Many proteins perform their function when attached to membrane surfaces. The binding of extrinsic proteins on nanodisc membranes can be indirectly imaged by transmission electron microscopy. We show that the characteristic stacking (rouleau) of nanodiscs induced by the negative stain sodium phosphotungstate is prevented by the binding of extrinsic protein.
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B. Kumar, R., Zhu, L., Hebert, H., Jegerschöld, C. Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy. J. Vis. Exp. (121), e55148, doi:10.3791/55148 (2017).
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