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Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis
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Biología
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JoVE Revista Biología
Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

DOI:
10.3791/55054-v

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07:40 min

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January 04, 2017

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, , ,
1Laboratory of Cell and Tissue Biology,Keio University School of Medicine, 2Department of Otolaryngology Head and Neck Surgery,Keio University School of Medicine

Capítulos

  • 00:05Título
  • 00:56Isolation of Auditory Bulla and Capsule
  • 02:25Isolation of Auditory Ossciles: Malleaus, Incus and Stapes
  • 04:25Preparation for Embedding in Frozen Blocks (Kawamoto’s Film Method)
  • 06:16Results: Histology of Paraffin and Frozen Sections
  • 06:57Conclusion

Summary

Traducción Automática

Traducción Automática

We present a protocol to isolate the auditory bulla, capsule, and ossicles from postnatal mice for whole mount and histological analysis.

Tags

Keywords: Auditory BullaMiddle Ear BonesHistological AnalysisPostnatal MiceWound BiologyMorphogenesisEndochondral OssificationMineral MetabolismAuditory OssiclesAuditory CapsuleSkull BonesBasisphenoidOccipital BoneInterparietalParietal BonesSquamosalFacial NerveVestibulo-cochlear NerveInternal Auditory CanalSubarcuate FossaAnterior Semicircular CanalMalleusIncusStapesTensor Tympani MuscleTympanic MembraneTympanic Bone

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