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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
Capítulos
- 00:05Título
- 00:47Culture Preparation
- 01:40Tissue Digestion: Mechanical and Enzymatic Dissociation
- 04:16Seeding of Cells
- 05:29Culture and Passaging of Cells
- 07:52Results: Human Primary Myoblasts Characterization and Its Use for Functional Studies of Muscle Homeostasis In Vitro
- 09:16Conclusion
This protocol describes a robust, reproducible and simple method of isolation and culture of myoblast progenitor cells from the skeletal muscle of adult and aged people. The muscles used here include foot and leg muscles. This approach enables the isolation of an enriched population of primary myoblasts for functional studies.
