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MicroRNA-based Regulation of Picornavirus Tropism
Capítulos
- 00:05Título
- 01:04Titrating Virus Stocks by Calculating 50% Tissue-culture Infectious Dose (TCID50)
- 02:33Evaluating MicroRNA-targeting Efficacy: Single-step Growth Kinetics
- 04:29Evaluating MicroRNA-targeting Specificity: Virus-spreading Assay Using Synthetic MicroRNA Mimics
- 07:01Results: Comparing MicroRNA-targeted Virus to Unmodified Virus
- 08:32Conclusion
We describe here a method for regulating picornavirus tropism by incorporating sequences complementary to specific microRNAs into the viral genome. This protocol can be adapted to all different classes of viruses with modifications based upon the length and nature of their life cycle.
