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JoVE Journal Chemistry
Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
 

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Article DOI: 10.3791/54975-v • 05:44 min • March 6th, 2017
March 6th, 2017 •
1, 1, 1
1Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem

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Here we present a protocol to measure the force of interactions between a well-defined inorganic surface and either peptides or amino acids by single-molecule force spectroscopy measurements using an atomic force microscope (AFM). The information obtained from the measurement is important to better understand the peptide-inorganic material interphase.

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Single-molecule Force Spectroscopy Adhesion Force Interactions Amino Acids Peptides Inorganic Materials Surface Chemistry Composites Chordates Silicon Nitride AFM Cantilevers Silicon Tips Ethanol Cleaning Oxygen Plasma Treatment Methyltriethoxysilane Three Amino Propyltriethoxysilane Inert Atmosphere Moisture Sensitivity Vacuum Pump
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