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dan Polyketomycin kullanma A Gösteri: Its Biyosentetik Gen kümesi için bir Doğal Ürün dan<em> Streptomyces diastatochromogenes</em> Tü6028
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Genética
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JoVE Revista Genética
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
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dan Polyketomycin kullanma A Gösteri: Its Biyosentetik Gen kümesi için bir Doğal Ürün dan<em> Streptomyces diastatochromogenes</em> Tü6028

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09:08 min

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January 13, 2017

DOI:

10.3791/54952-v

DOI:
10.3791/54952-v

•

09:08 min
January 13, 2017

•

12 Views
, , ,
1Department of Pharmaceutical Biology and Biotechnology,Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology,Universität Siegen

Summary

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Burada, (A), antibiyotik aktivitesi ile doğal ürünün belirlenmesi, (B) bileşiği ile saflaştırılması, (c) biyosentezinin ilk modeli, (D), genom dizileme / -Maden ve detaylı bir protokol mevcut ( E) biyosentetik gen kümesinin doğrulanması.

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Greule, A., Zhang, S., Paululat, T., Bechthold, A. From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028. J. Vis. Exp. (119), e54952, doi:10.3791/54952 (2017).

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