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Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion
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Medicina
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JoVE Revista Medicina
Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion

Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion

8,413 Views

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08:22 min

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March 20, 2017

DOI:

10.3791/54912-v

DOI:
10.3791/54912-v

•

08:22 min
March 20, 2017

•

3 Views
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1Laboratory for Physiology, Institute for Cardiovascular Research (ICaR-VU),VU University Medical Center, 2Department of Internal Medicine, Institute for Cardiovascular Research (ICaR-VU),VU University Medical Center

Tags

Intravital MicroscopyContrast-enhanced UltrasonographyMouse HindlimbInsulin-induced VasodilationMuscle PerfusionType 2 DiabetesObesityInsulin SensitivityOrgan Blood FlowCD57 BL6 Male MouseAdductor CompartmentFemoral ArteryEpigastric ArteryGracilis ArteryDeep FasciaParaffin Oil

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Turaihi, A. H., van Poelgeest, E. M., van Hinsbergh, V. W. M., Serné, E. H., Smulders, Y. M., Eringa, E. C. Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion. J. Vis. Exp. (121), e54912, doi:10.3791/54912 (2017).

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