Journal
/
Biología
/
LAP-tagged מושרה שורות תאים יציבות על תפקוד החלבון חוקר, Spatiotemporal לוקליזציה רשתות אינטראקציה חלבון
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
Please note that all translations are automatically generated. Click here for the English version.

LAP-tagged מושרה שורות תאים יציבות על תפקוד החלבון חוקר, Spatiotemporal לוקליזציה רשתות אינטראקציה חלבון

9,630 Views

•

11:04 min

•

December 24, 2016

DOI:

10.3791/54870-v

DOI:
10.3791/54870-v

•

11:04 min
December 24, 2016

•

2 Views
, , , ,
1Department of Chemistry and Biochemistry,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles, 3Jonsson Comprehensive Cancer Center,University of California, Los Angeles

Summary

Automatically generated

We describe a method for generating localization and affinity purification (LAP)-tagged inducible stable cell lines for investigating protein function, spatiotemporal subcellular localization and protein-protein interaction networks.

Explore More Videos

Inducible LAP-taggedStable Cell LinesProtein FunctionSpatiotemporal LocalizationProtein Interaction NetworksHEK293 CellsHygromycin SelectionTandem Affinity Purification (TAP)Tet/Dox InductionAnti-GFP AntibodyProtein A Beads

Read Article

Cite this Article

Bradley, M., Ramirez, I., Cheung, K., Gholkar, A. A., Torres, J. Z. Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks. J. Vis. Exp. (118), e54870, doi:10.3791/54870 (2016).

Download .ris file

Copy

Share Video

.

Copy