Journal
/
Neurociencias
/
用于访问视网膜下腔的另类和验证注射法<i>通过</i>一经巩膜后路
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
Please note that all translations are automatically generated. Click here for the English version.

用于访问视网膜下腔的另类和验证注射法<i>通过</i>一经巩膜后路

12,890 Views

•

07:13 min

•

December 07, 2016

DOI:

10.3791/54808-v

DOI:
10.3791/54808-v

•

07:13 min
December 07, 2016

•

6 Views
, , , , ,
1Jules Stein Eye Institute,University of California, Los Angeles

Summary

Automatically generated

Subretinal injections are the most common technique for delivering large therapeutic agents such as proteins and viral vectors to photoreceptors and the retinal pigment epithelium. An alternative method in mice that successfully targets the subretinal space with minimal collateral damage and fast recovery times is described here.

Explore More Videos

Subretinal InjectionTranscleral Posterior ApproachViral VectorsPharmacological AgentsPluripotent Stem CellsRetinal DegenerationFluoresceinAnesthetized MousePupil DilationScleral IncisionOphthalmic Blade33-gauge NeedleMinimally InvasiveRetinal Structure And Function

Read Article

Cite this Article

Parikh, S., Le, A., Davenport, J., Gorin, M. B., Nusinowitz, S., Matynia, A. An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach. J. Vis. Exp. (118), e54808, doi:10.3791/54808 (2016).

Download .ris file

Copy

Share Video

.

Copy