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在动物标本呼吸道病原体的高通量检测通过纳米PCR
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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在动物标本呼吸道病原体的高通量检测通过纳米PCR

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11:00 min

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November 28, 2016

DOI:

10.3791/54781-v

DOI:
10.3791/54781-v

•

11:00 min
November 28, 2016

•

2 Views
, , , , , , , , ,
1Population Medicine and Diagnostic Sciences,Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.

Summary

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的DNA和RNA基于病原体由纳米级的PCR的高通量的检测是使用综合征犬和马呼吸PCR面板说明。

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Keyword Extraction:High-throughput DetectionRespiratory PathogensAnimal SpecimensNanoscale PCRReal-time PCRDNARNAPathogensOneHealth SurveillanceInfectious DiseasesClinical SpecimensRespiratory PathogensHorsesDogsLysis BufferReverse TranscriptionPre-amplificationPreamp96-well PlateThermocyclerCDNA Production

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Cite this Article

Goodman, L. B., Anderson, R. R., Slater, M., Ortenberg, E., Renshaw, R. W., Chilson, B. D., Laverack, M. A., Beeby, J. S., Dubovi, E. J., Glaser, A. L. High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR. J. Vis. Exp. (117), e54781, doi:10.3791/54781 (2016).

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