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İrritabl Bağırsak Sendromu içinde miRNA'lar Sirkülasyon tedirginlikler bir Çoklanmış Yüksek verim Gen İfadesi Platformu Kullanma Algılanan
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Genética
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JoVE Revista Genética
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
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İrritabl Bağırsak Sendromu içinde miRNA'lar Sirkülasyon tedirginlikler bir Çoklanmış Yüksek verim Gen İfadesi Platformu Kullanma Algılanan

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10:37 min

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November 30, 2016

DOI:

10.3791/54693-v

DOI:
10.3791/54693-v

•

10:37 min
November 30, 2016

•

1 Views
, , , , ,
1Digestive Disorders Unit, National Institute of Nursing Research,National Institutes of Health, DHHS, 2National Institutes of Health Research Scholar,Howard Hughes Medical Institute, 3Internal Medicine, Medical School,University of Michigan

Summary

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We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.

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Cite this Article

Fourie, N. H., Peace, R. M., Abey, S. K., Sherwin, L. B., Wiley, J. W., Henderson, W. A. Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform. J. Vis. Exp. (117), e54693, doi:10.3791/54693 (2016).

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