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Messung von Influenza-Neuraminidase Inhibition Antikörpertiter durch Enzyme-Linked-Lectin-Assay
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
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Messung von Influenza-Neuraminidase Inhibition Antikörpertiter durch Enzyme-Linked-Lectin-Assay

17,844 Views

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08:11 min

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September 06, 2016

DOI:

10.3791/54573-v

DOI:
10.3791/54573-v

•

08:11 min
September 06, 2016

•

39 Views
, ,
1Division of Viral Products, CBER,Food and Drug Administration

Summary

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We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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InfluenzaNeuraminidaseAntibodyELLAEnzyme-linked Lectin AssayH6 Reassortant VirusFetuin-coated PlatePeanut AgglutininHRPVaccine

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Gao, J., Couzens, L., Eichelberger, M. C. Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay. J. Vis. Exp. (115), e54573, doi:10.3791/54573 (2016).

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