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JoVE Journal Genetics
Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
 

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Article DOI: 10.3791/54528-v • 09:51 min • October 1st, 2016
October 1st, 2016 •
1, 1
1Department of Biology and Biotechnology, University of Pavia

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Resumen
Automatic Translation

The Mediterranean fruit fly (medfly) Ceratitis capitata (Diptera: Tephritidae) is a worldwide pest of agriculture. A deeper understanding of its biology is key to control medfly populations and thus reduce economic impact. Embryo microinjection is a fundamental tool allowing both germ-line transformation and reverse genetics studies in this species.

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Nucleic Acids Embryo Microinjection Ceratitis Capitata Agricultural Pest Insect Toolbox For Control Functional Gene Correction Transgenic Strains Low Amount Of Nucleic Acids Medfly Colony Temperature And Humidity Conditions Light/dark Photo-period Water Supply Through Capillary Action Cage Setup Pupae Loading Food Mixture Of Yeast And Sugar Egg Collection And Fertilization Larval Food
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