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使用新型不溶性生色底物检测试剂盒碳水化合物降解酶的高通量筛选
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Bioquímica
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JoVE Revista Bioquímica
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
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使用新型不溶性生色底物检测试剂盒碳水化合物降解酶的高通量筛选

12,518 Views

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06:51 min

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September 20, 2016

DOI:

10.3791/54286-v

DOI:
10.3791/54286-v

•

06:51 min
September 20, 2016

•

11 Views
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1Department for Plant and Environmental Sciences,University of Copenhagen, 2School of Agriculture, Food and Rural Development,Newcastle University

Tags

High-throughput ScreeningCarbohydrate-degrading EnzymesNovel Insoluble Chromogenic Substrate Assay KitsEnzyme DiscoveryBiomass DegradationChromogenic SubstratesEndo-cellulaseICB-wheat StrawAbsorbancePlate Reader

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Schückel, J., Kračun, S. K., Willats, W. G. T. High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits. J. Vis. Exp. (115), e54286, doi:10.3791/54286 (2016).

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