Journal
/
Biología del desarrollo
/
结合活体荧光显微镜 (IVFM) 和遗传模型研究造血细胞对骨髓生态位的植入动力学
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
Please note that all translations are automatically generated. Click here for the English version.

结合活体荧光显微镜 (IVFM) 和遗传模型研究造血细胞对骨髓生态位的植入动力学

DOI:
10.3791/54253-v

•

11:06 min

•

March 21, 2017

•

, , ,
1Wells Center for Pediatric Research, Department of Pediatrics,Indiana University School of Medicine, 2Indiana Center for Biological Microscopy, Department of Medicine,Indiana University School of Medicine, 3Department of Pediatrics,Indiana University School of Medicine

Capítulos

  • 00:05Título
  • 01:26Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
  • 03:16Lys-GFP BMT and Intravital Imaging Preparation
  • 05:23In Vivo High Resolution Mouse Calvarium Imaging
  • 07:15Quantitative Analysis
  • 08:14Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
  • 10:10Conclusion

Summary

Traducción Automática

Traducción Automática

活体荧光显微镜 (IVFM) 的颅骨是应用与遗传动物模型, 研究造血细胞归巢和植入的骨髓 (BM) 龛。

Tags

Intravital Fluorescent MicroscopyIVFMHematopoietic Cell EngraftmentBone Marrow NichesHematopoietic Cell RegenerationCell-to-cell InteractionsMyeloproliferative DiseaseNotch SignalingEGFP MouseBone Marrow TransplantCell IsolationCell SuspensionTail Vein Injection

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

造血干细胞的自动定量 - 在脱钙骨的组织学图像间质细胞的相互作用

酶法从凝块骨髓样本拯救间充质干细胞

从人骨髓分离Mesangiogenic祖细胞（多用途储值卡）

在体外和体内模型来研究角膜内皮-间充质转换

RANKL 小鼠骨髓破骨细胞培养法研究 mTORC1 在破骨细胞形成中的作用

小鼠骨髓基质细胞与骨破骨祖的分离、培养及分化

秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

成人斑马鱼损伤模型研究泼尼松对再生骨组织的影响

评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C） 损伤的反应

杏中自体和（内）相容关系的确定结合手授、显微镜和遗传分析

Read Article