Journal
/
Biología del desarrollo
/
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

11,953 Views

•

06:02 min

•

June 10, 2016

DOI:

10.3791/53974-v

DOI:
10.3791/53974-v

•

06:02 min
June 10, 2016

•

6 Views
, , , , ,
1Department of Clinical Laboratory,The University of Tokyo Hospital, 2Department of Respiratory Medicine,The University of Tokyo Hospital

Summary

Automatically generated

Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.

Explore More Videos

In Vitro AssayMesenchymal CellsEpithelial-mesenchymal Transition (EMT)ContractilityInflammatory CytokinesIdiopathic Pulmonary FibrosisAirway RemodelingA-549 CellsTGF-beta1TNF-alphaGel Medium3D Culture

Read Article

Cite this Article

Mikami, Y., Matsuzaki, H., Takeshima, H., Makita, K., Yamauchi, Y., Nagase, T. Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition. J. Vis. Exp. (112), e53974, doi:10.3791/53974 (2016).

Download .ris file

Copy

Share Video

.

Copy