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Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
JoVE Revista
Biología del desarrollo
This content is Free Access.
JoVE Revista Biología del desarrollo
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

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13:01 min

April 10, 2016

DOI:

13:01 min
April 10, 2016

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Summary

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Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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