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Epigenetisk Konvertering som en sikker og enkel metode til at opnå insulinproducerende celler fra voksne hud fibroblaster
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Epigenetisk Konvertering som en sikker og enkel metode til at opnå insulinproducerende celler fra voksne hud fibroblaster

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08:45 min

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March 18, 2016

DOI:

10.3791/53880-v

DOI:
10.3791/53880-v

•

08:45 min
March 18, 2016

•

3 Views
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1Laboratory of Biomedical Embryology, Unistem, Centre for Stem Cell Research,Università degli Studi di Milano

Tags

Epigenetic ConversionInsulin-secreting CellsAdult Skin FibroblastsRegenerative MedicineCell TherapyDiabetesPluripotencyRetroviral VectorsPorcine GelatinPBSAntibiotic AntimycoticSkin BiopsyFibroblast MediumCell MonolayerPassage RatioTrypsin-EDTACell SuspensionCounting Chamber

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Brevini, T. A., Pennarossa, G., Maffei, S., Zenobi, A., Gandolfi, F. Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts. J. Vis. Exp. (109), e53880, doi:10.3791/53880 (2016).

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