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RNAi Trigger Delivery into Anopheles gambiae Pupae
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March 08, 2016
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Summary
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RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development.
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Regna, K., Harrison, R. M., Heyse, S. A., Chiles, T. C., Michel, K., Muskavitch, M. A. T. RNAi Trigger Delivery into Anopheles gambiae Pupae. J. Vis. Exp. (109), e53738, doi:10.3791/53738 (2016).
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