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益生菌酵母的转型及其胃肠道粘膜免疫组织恢复继小鼠口服灌胃
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
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益生菌酵母的转型及其胃肠道粘膜免疫组织恢复继小鼠口服灌胃

DOI:
10.3791/53453-v

•

12:12 min

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February 08, 2016

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, , , ,
1Department of Pediatrics,Emory University School of Medicine, 2Department of Biochemistry,Emory University School of Medicine

Capítulos

  • 00:05Título
  • 01:00Generate Survival Curves to Determine Needed Doses of UV Irradiation
  • 03:01Screening for Mutagenized Auxotrophic Yeast Strains and Yeast Transformation
  • 05:42Oral Gavage of Mice with Transformed Yeast
  • 07:50Harvest of Murine Peyer’s Patches and Isolation of Viable Yeast Colonies
  • 10:03Representative Results
  • 11:28Conclusion

Summary

Traducción Automática

Traducción Automática

这里介绍的是，可以用于开发，变换，管理，和测试益生菌酵母布拉酵母菌中异源蛋白质表达技术的统一描述。

Tags

Probiotic YeastOral GavageUV Irradiation5-FOAUra3 MutantOxytrophic StrainsGenetic ManipulationOral VaccinationGastrointestinal Therapeutics

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