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Optimering af såret Scratch-analysen til at påvise ændringer i murine Mesenchymale stromacelle Migration efter skade ved Opløselig Cigaret røgekstrakt
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Capítulos
- 00:05Título
- 00:40Creating the Scratch
- 02:51Taking Initial Images of the Scratch
- 04:34Analyzing Initial Scratch Images
- 06:07Taking Final Images of the Scratch and Analyzing Final Scratch Images
- 06:52Results: Migratory of Murine Lung MSCs is Altered After Damage with Cigarette Smoke Extract
- 08:02Conclusion
The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.
