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Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

13,265 Views

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10:39 min

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October 30, 2015

DOI:

10.3791/53216-v

DOI:
10.3791/53216-v

•

10:39 min
October 30, 2015

•

2 Views
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1The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, 2The Metabolic Phenotyping Core,Virginia Tech, 3Seahorse Bioscience

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Key Words:Mitochondrial RespirationSkeletal MuscleMouseMicroplateHigh-throughputIsolated MitochondriaRespiratory Measurements

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Boutagy, N. E., Rogers, G. W., Pyne, E. S., Ali, M. M., Hulver, M. W., Frisard, M. I. Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements. J. Vis. Exp. (104), e53216, doi:10.3791/53216 (2015).

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