Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Purification of Mouse Brain Vessels
JoVE Journal
Neuroscience

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Neuroscience
Purification of Mouse Brain Vessels
 

Purification of Mouse Brain Vessels

Article DOI: 10.3791/53208-v • 07:32 min • November 10th, 2015
November 10th, 2015 •
1,2,3, 4, 4, 1,2,3
1Collège de France, Center for Interdisciplinary Research in Biology (CIRB), Centre National de la Recherche Scientifique CNRS, Unité Mixte de Recherche 7241, Institut National de la Santé et de la Recherche Médicale, 2Centre Interdisciplinaire de Recherche en Biologie, 3MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University, 4Université Paris Descartes, Faculté de Pharmacie, Université Paris Diderot

Capítulos

Resumen
Automatic Translation

We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.

Tags

Keywords: Brain Vasculature Blood-brain Barrier Brain Pathologies Cerebrovascular Physiology Primary Culture Cell Sorting Brain Vessel Purification Endothelial Cells Tight Junctions Basal Lamina Pericytes Smooth Muscle Cells Astrocyte Endfeet Immunostaining
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter