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树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生
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Biología del desarrollo
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Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
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树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生

11,236 Views

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10:18 min

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October 10, 2015

DOI:

10.3791/53197-v

DOI:
10.3791/53197-v

•

10:18 min
October 10, 2015

•

8 Views
, , , , ,
1Human Genetics and Cognitive Functions,Institut Pasteur, 2CNRS URA 2182 ‘Genes, synapses and cognition’,Institut Pasteur, 3Human Genetics and Cognitive Functions,Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d’ Imagerie Dynamique, Imagopole, CiTech,Institut Pasteur, 5Neuroplasticity and Therapeutics,CECS, I-STEM, AFM, Evry

Summary

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锥体神经元的树突棘是哺乳动物大脑皮质最兴奋性突触的部位。此方法描述在从诱导的多能干细胞衍生的人皮质锥体谷氨酸能神经元脊柱形态的三维定量分析。

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Gouder, L., Tinevez, J., Goubran-Botros, H., Benchoua, A., Bourgeron, T., Cloëz-Tayarani, I. Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (104), e53197, doi:10.3791/53197 (2015).

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