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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
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October 31, 2015
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Preparation of protein-functionalized planar glass-supported lipid bilayers, determination of protein mobility within and measurement of protein densities is shown here. A roadmap to building a noise-reduced Total Internal Reflection microscope is outlined, which allows visualizing single bilayer-resident fluorochromes with high spatiotemporal resolution.
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Axmann, M., Schütz, G. J., Huppa, J. B. Single Molecule Fluorescence Microscopy on Planar Supported Bilayers. J. Vis. Exp. (104), e53158, doi:10.3791/53158 (2015).
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