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性T细胞Transinfection从树突状细胞捕捉细菌

JoVE Revista
Inmunología y contagio

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JoVE Revista Inmunología y contagio

T Cells Capture Bacteria by Transinfection from Dendritic Cells

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性T细胞Transinfection从树突状细胞捕捉细菌

11,545 Views

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11:39 min

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January 13, 2016

DOI:

10.3791/52976-v

DOI:

10.3791/52976-v

•

11:39 min

January 13, 2016

•

1 Views

Aranzazu Cruz-Adalia¹, Guillermo Ramírez-Santiago^1,2, Mónica Torres-Torresano^1,2, Raquel Garcia-Ferreras^1,2, Esteban Veiga Chacón¹

¹Cellular and Molecular Biology, Spanish National Research Council (CSIC),Spanish National Biotechnology Centre (CNB-CSIC), ²Hospital de Santa Cristina,Healthcare Research Institute Princesa Hospital (IIS-Princesa)

Summary

这里的协议，提出通过该期间抗原呈递发生经由transinfection从预感染树突状细胞（DC）的CD4 ^{+ T细胞}以测量细菌捕获。我们说明如何执行必要的步骤：原代细胞中，DC，DC / T细胞共轭形成感染和细菌的T细胞转染的测量隔离。

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