Journal
/
Biología
/
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
JoVE Revista
Biología
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

DOI:
10.3791/52824-v

•

07:48 min

•

July 03, 2015

•

,
1Department of Anesthesiology and Perioperative Care,University of California Irvine, 2Department of Biomedical and Molecular Sciences,Queen’s University, 3Department of Pharmacology,University of California Irvine

Capítulos

  • 00:05Título
  • 01:25Drug Treatment of Cultured Cells and Immunocytochemistry
  • 03:26Microscope Settings and Imaging
  • 04:57Colocalization Analysis and Data Normalization
  • 06:17Results: Representative Photomicrographs of Receptor and Compartment-marker Labeling with False-color Colocalization Maps
  • 07:18Conclusion

Summary

Traducción Automática

Traducción Automática

Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.

Tags

Receptor TraffickingReceptor InternalizationColocalization AnalysisImmunolabelingConfocal MicroscopyDrug-induced ChangesCell SignalingIntracellular TraffickingQuantitative Analysis

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

Environmentally Induced Heritable Changes in Flax

Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Read Article