This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.
Ramond, C., Bandeira, A., Berthault, C., Pereira, P., Cumano, A., Burlen-Defranoux, O. Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays. J. Vis. Exp. (100), e52795, doi:10.3791/52795 (2015).