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Immunology and Infection

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JoVE Journal Immunology and Infection
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
 

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Article DOI: 10.3791/52687-v • 09:43 min • April 16th, 2015
April 16th, 2015 •
*1, *1, 1, 1, 1
1LD MacLean Surgical Research Laboratories, Department of Surgery, McGill University
* These authors contributed equally

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Resumen
Automatic Translation

In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.

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Keywords: Neutrophil Extracellular Traps (NETs) NET Isolation NET Handling Cell-free NET Isolation NET-based Assays NET Adhesion Assay A549 Lung Cancer Cells PMA Stimulation Density Gradient Separation DNase1
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