Journal
/
Neurociencias
/
Perforert Patch-clamp Opptak av Mouse olfactory sensoriske nevroner i Intakt neuroepithelium: Funksjonell analyse av nerveceller uttrykker en Identifiserte odoranten Receptor
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Perforert Patch-clamp Opptak av Mouse olfactory sensoriske nevroner i Intakt neuroepithelium: Funksjonell analyse av nerveceller uttrykker en Identifiserte odoranten Receptor

DOI:
10.3791/52652-v

•

10:16 min

•

July 13, 2015

•

,
1UMR Centre des Sciences du Goŭt et de l’Alimentation, CNRS, INRA,Université de Bourgogne

Capítulos

  • 00:05Título
  • 01:14Pipette and Solution Preparations
  • 03:45Preparing the Olfactory Epithelium
  • 06:58Making Recordings
  • 08:47Results: Stimulating the MOR23 Receptor of Olfactory Receptor Neurons (ORNs) with Lyral
  • 09:43Conclusion

Summary

Traducción Automática

Traducción Automática

Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors.

Tags

Keywords: Olfactory Sensory NeuronsPatch-clamp RecordingOdorant ReceptorGenetically Tagged MiceGFPOlfactory EpitheliumMembrane PropertiesLigand-receptor InteractionsTransduction PathwaysPharmacologyCoding Properties

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/52652?article_type=v

Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording
check_url/es/52652?article_type=v

Flash Fotolyse av Caged forbindelser i Cilia av olfactory sensoriske nevroner
check_url/es/52652?article_type=v

Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
check_url/es/52652?article_type=v

Retrograde Fluorescent Merking Tillater målrettet Ekstracellulær Single-enhet Opptak fra Identifiserte Nerveceller<em> In vivo</em
check_url/es/52652?article_type=v

Innspilling elektriske aktiviteten fra Identifiserte Nerveceller i Intakt Norgesmester Transgenic Fish
check_url/es/52652?article_type=v

Isolering av sensoriske nevroner av<em> Aplysia californica</em> For Patch Clamp Recordings i glutamaterg Currents
check_url/es/52652?article_type=v

Intracellulær opptak, Sensory felttilordningen, og Dyrknings Identifiserte Nerveceller i Leech, Hirudo medicin</i
check_url/es/52652?article_type=v

Hele Mount immunolabeling av olfactory Receptor nevroner i<em> Drosophila</em> Antenne
check_url/es/52652?article_type=v

Innspilling Lys-fremkalt postsynaptiske Responses i nevroner i Dark-tilpasset, Mouse Netthinne Slice Forberedelser Bruke Patch Clamp Teknikker
check_url/es/52652?article_type=v

Forberedelser og protokoller for hele cellen Patch klemme opptak i Xenopus laevis Tectal nerveceller

Read Article