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EPA Método 1615. Medição de Enterovirus e Norovirus Ocorrência na água por cultura e RT-qPCR. Parte III. Detecção de vírus por RT-qPCR
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Capítulos
- 00:05Título
- 02:03Tertiary Concentration and Nucleic Acid Isolation
- 03:21Reverse Transcription
- 05:57Real Time Quantitative PCR
- 09:23Results: RT-qPCR Determination of Enterovirus and Norovirus in Water
- 11:38Conclusion
Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.
