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엔테로 바이러스 및 문화와 RT-qPCR에 의해 물에서 노로 바이러스의 발생의 EPA 방법 1615 측정. 파트 III. RT-qPCR에 의한 바이러스 검출
JoVE Revista
Medio ambiente
This content is Free Access.
JoVE Revista Medio ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
DOI:

12:32 min

January 16, 2016

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Capítulos

  • 00:05Título
  • 02:03Tertiary Concentration and Nucleic Acid Isolation
  • 03:21Reverse Transcription
  • 05:57Real Time Quantitative PCR
  • 09:23Results: RT-qPCR Determination of Enterovirus and Norovirus in Water
  • 11:38Conclusion

Summary

Traducción Automática

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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