Journal
/
Biología del desarrollo
/
协议获得合子和体细胞胚胎研究早期胚胎发育的调控中的示范豆科<em>苜蓿</em
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
Please note that all translations are automatically generated. Click here for the English version.

协议获得合子和体细胞胚胎研究早期胚胎发育的调控中的示范豆科<em>苜蓿</em

13,725 Views

•

07:32 min

•

June 09, 2015

DOI:

10.3791/52635-v

DOI:
10.3791/52635-v

•

07:32 min
June 09, 2015

•

2 Views
, , , , ,
1School of Environmental and Life Sciences,University of Newcastle, Callaghan, New South Wales, Australia

Summary

Automatically generated

The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.

Explore More Videos

Zygotic EmbryosSomatic EmbryosMedicago TruncatulaEarly Embryo DevelopmentEmbryogenesisPod MorphologyLeaf ExplantsAuxinCytokininAbscisic AcidGibberellic Acid

Read Article

Cite this Article

Kurdyukov, S., Song, Y., Tiew, T. W., Wang, X., Nolan, K. E., Rose, R. J. Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula. J. Vis. Exp. (100), e52635, doi:10.3791/52635 (2015).

Download .ris file

Copy

Share Video

.

Copy