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Immunology and Infection

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JoVE Journal Immunology and Infection
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
 

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Article DOI: 10.3791/52610-v • 11:19 min • May 4th, 2015
May 4th, 2015 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 3,4, 1,2
1Department of Microbiology, University of Washington, 2Departments of Medicine and Laboratory Medicine, University of Washington, 3U.S Military HIV Research Program, Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

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Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

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Pairwise Growth Competition Assay Replication Fitness Human Immunodeficiency Viruses In Vitro Fitness Assay Viral Replication Fitness HIV-1 Viral Particles Per Infectious Unit Growth Rate In Cell Culture Relative Fitness Multiple-cycle Growth Competition Assay Sensitive Measurement Of Fitness Cellular Targets Growth Conditions Experimental Factors Multiplicity Of Infection (MOI) Sampling Times Viral Detection Methods Fitness Calculation Methods Recombinant HIV-1 Clone Experimental Results Analysis
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