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Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
JoVE Revista
Medicina
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JoVE Revista Medicina
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

DOI:
10.3791/52599-v

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08:34 min

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April 13, 2015

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, , , , , ,
1Department of Cancer Biology,UT MD Anderson Cancer Center, 2Novartis Institutes for Biomedical Research, 3Sanofi US, 4Institute of Applied Cancer Science,UT MD Anderson Cancer Center

Capítulos

  • 00:05Título
  • 01:35Reactivation of Telomerase in mTERT-ER and LSL-mTERT Mice In Vivo
  • 02:33Isolation of Neural Stem Cells and Reactivation of Telomerase In Vitro
  • 05:07Telomere FISH
  • 06:37Results: Effect of Telomerase Reactivation
  • 08:01Conclusion

Summary

Traducción Automática

Traducción Automática

Telomere and telomerase play essential roles in ageing and tumorigenesis. The goal of this protocol is to show how to generate two murine inducible telomerase knock-in alleles and how to utilize them in the studies of tissue degeneration/regeneration and cancer.

Tags

Telomere DysfunctionTelomerase ReactivationCancerNeural Stem CellsMurine Models4-Hydroxytamoxifen (4-OHT)Lox-Stopper-LoxTERT (LSL-mTERT)Telomere FISHMetaphase Chromosomes

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