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Türler özgü Algılama Loop-aracılı İzotermal Amplifikasyon (LAMP) Tahliller<em> Eimeria</em> Tavuklar bulaştırmak ki
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
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Türler özgü Algılama Loop-aracılı İzotermal Amplifikasyon (LAMP) Tahliller<em> Eimeria</em> Tavuklar bulaştırmak ki

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06:57 min

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February 20, 2015

DOI:

10.3791/52552-v

DOI:
10.3791/52552-v

•

06:57 min
February 20, 2015

•

5 Views
, , ,
1Department of Pathology and Pathogen Biology,Royal Veterinary College, London, 2BIOPHARM,Research Institute of Biopharmacy and Veterinary Drugs

Summary

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Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.

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Barkway, C. P., Pocock, R. L., Vrba, V., Blake, D. P. Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens. J. Vis. Exp. (96), e52552, doi:10.3791/52552 (2015).

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