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Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
Chapters
- 00:05Title
- 01:16Template Preparation
- 02:44Eimeria LAMP Primer Preparation
- 03:50Eimeria LAMP Assay and Read-out
- 05:22Results: Eimeria LAMP Assay Testing
- 06:30Conclusion
Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.
