La inducción de la inflamación intestinal murino por transferencia adoptiva de Efector CD4<sup> +</sup> CD45RB<sup> Alta</sup> T células en ratones inmunodeficientes
La inducción de la inflamación intestinal murino por transferencia adoptiva de Efector CD4<sup> +</sup> CD45RB<sup> Alta</sup> T células en ratones inmunodeficientes
Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.
Steinbach, E. C., Gipson, G. R., Sheikh, S. Z. Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice. J. Vis. Exp. (98), e52533, doi:10.3791/52533 (2015).