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Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures
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Capítulos
- 00:05Título
- 01:23Plethysmograph Chamber Set Up and Cycle Time Calibration
- 02:54Acute Intermittent Hypoxia Administration and Subventricular Zone Stem/Progenitor Cell Culture
- 04:33Results: Representative Neurosphere Development
- 05:17Conclusion
This article describes the methodology for administering short periods of intermittent hypoxia to postnatal day 1-8 mouse or rat pups. This approach effectively elicits a robust tissue level “priming effect” on cultured neural progenitor cells that are harvested within 30 min of hypoxia exposure.
