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At analysere virkningen af ​​stromacellers om ansættelse af Leukocytter fra Flow
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
DOI:

11:30 min

January 07, 2015

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Capítulos

  • 00:05Título
  • 01:28Assembling the Flow System
  • 04:44Setting Up Microslides and the Parallel Plate Flow Chamber Incorporating Filters for Flow
  • 06:59Perfusion of Leukocytes Over Endothelial Cells
  • 07:55Recording Neutrophil Capture and Behavior
  • 09:47Results: Evaluation of Neutrophil Recruitment to Endothelial Cells Cultured Alone or with MSC
  • 11:06Conclusion

Summary

Traducción Automática

Evne betændt endotel at rekruttere leukocytter fra flow reguleres af mesenkymale stromale celler. Vi beskriver to in vitro modeller med primære humane celler, som kan anvendes til at vurdere neutrofil rekruttering fra flow og undersøge den rolle, som mesenchymale stromale celler spiller i reguleringen af denne proces.

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