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Um protocolo simples e rápido para não enzimática Separar frescos Tecidos Humanos para a Análise de infiltrar Linfócitos
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Capítulos
- 00:05Título
- 01:47Preparation of the Tissue Homogenate
- 04:15Separation and Clarification of the Tissue Supernatant
- 05:37Results: Tumor Infiltrating Lymphocyte (TIL) Subsets in the Homogenate
- 07:00Conclusion
This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.
